Defined medium for growth of Haemophilus influenzae.
نویسندگان
چکیده
A chemically defined medium (MI(c)) is described in which cells of Haemophilus influenzae Rd grow rapidly (generation time, 35 +/- 5 min) and reach a stationary level of 10(10) cells/ml. Our strain of cells grown in this medium developed high levels of competence when transferred to another medium designed for that purpose. Conditions governing the total development of competence in this organism have now been defined.
منابع مشابه
Improvement of Large-scale PRP production by Haemophilus influenzae typeb, using modified CY medium
Background and Objective: Haemophilus influenzae type b (Hib) is a gram negative bacterium and one of the most common causative agents of acute meningitis in infants and less than 5 years old children worldwide. The production of Hib capsular polysaccharide polyribosyl ribitolphosphate (PRP) is important for the production of conjugate vaccines against Hib infections. The aim of this study is t...
متن کاملA study on Haemophilus influenzae type b growth rate and capsule production in different media
In the present study, seven isolates of Haemophilus influenzae type b were cultured in four different media to compare growth rate and capsule production. Four liquid media namely brain heart infusion broth (BHI), trypticase soy broth (TSB), Mueller Hinton Broth (MHB) and Gonococci broth (GC) with added supplements (1% hemoglobin, 1% Isovitalex) were used. The growth was measured by colony coun...
متن کاملIron acquisition by Haemophilus influenzae.
The mechanisms for acquisition of iron by Haemophilus influenzae and their role in pathogenesis are not known. Heme and nonheme sources of iron were evaluated for their effect on growth of type b and nontypable strains of H. influenzae in an iron-restricted, defined medium. All 13 strains acquired iron from heme, hemoglobin, hemoglobin-haptoglobin, and heme-hemopexin. Among nonheme sources of p...
متن کاملElemental iron does repress transferrin, haemopexin and haemoglobin receptor expression in Haemophilus influenzae.
The iron repressible nature of Haemophilus influenzae transferrin binding proteins suggests a regulatory role for elemental iron in their expression. The existence of a Haemophilus ferric uptake repressor (Fur) binding motif identified in the promoter region of both tbpA and tbpB further supports this hypothesis. However, a recent study using brain heart infusion growth medium suggested that tr...
متن کاملCarriage Rate of Nasopharyngeal Haemophilus Influenzae among Children under 6 Years Old in Tehran, Iran
Introduction : Haemophilus influenzae is a gram-negative bacterium causing a variety of respiratory infections in developing countries, especially in children. Nasopharynx carriers of H. influenzae are the prominent source and transitional vectors of invasive diseases. As very limited information on H. influenzae carriage rate in Iran was available, an evaluation on prevalence of this bacteri...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 101 2 شماره
صفحات -
تاریخ انتشار 1970